Journal: Scientific Reports
Article Title: Ablation of lipocalin-2 reduces neuroinflammation in a mouse model of Krabbe disease
doi: 10.1038/s41598-024-82927-1
Figure Lengend Snippet: Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) Proteome Profiler Mouse Cytokine Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Article Snippet: The Proteome Profiler Mouse XL Cytokine Array analysis (R&D Systems #ARY028) was performed on whole brain lysates as previously described .
Techniques: Immunohistochemistry, Quantitative RT-PCR